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Antibodies
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) antibody
Enolase 2 (Gamma, Neuronal) (ENO2) antibody
| Antigen |
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| Synonyms |
ENO2, DKFZp459B1817, NSE, Eno-2, AI837106, D6Ertd375e, RNEN3, Lrpb7 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
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| Catalog no. |
ABIN343446 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Neuron specific enolase, NSE
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Immunogen
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A peptide mapping at the C-terminus of NSE of human origin, identical to the related mouse and rat sequence.
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Format
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Affinity-purified
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Isotype
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IgG (Matching secondary antibodies)
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Description
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NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
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Specificity
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Human, mouse, rat. No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 1-2u/ml with the appropriate system to detect NSE in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect NSE in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.
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Purity
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Immunogen affinity purified.
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Buffer
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Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Restrictions
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For Research Use only
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Alternatives for antigen "Enolase 2 (Gamma, Neuronal) (ENO2)", type "Antibodies"
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Hosts
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Mouse (60), Rabbit (22), Chicken (1), Sheep (1)
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Reactivities
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Human (69), Guinea Pig (7), Mouse (Murine) (6), Rat (Rattus) (6), Chicken (2), Dog (Canine) (2), Cow (Bovine) (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Enzyme Immunoassay (EIA) (28), ELISA (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (8), Immunofluorescence (IF) (7), ELISA (Detection) (5), ELISA (Capture) (4)
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Epitopes
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pTyr236 (1)
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