Nogo A antibody

Antigen: Nogo A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343447

Additional Information

Alternative name: NOGO-A

Immunogen: A synthetic peptide corresponding to the C-terminal of human Nogo-A, identical to the related mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect Nogo-A in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect Nogo-A in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of rat brain tissue lysis using NOGO-A antibody