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Antibodies
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anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) antibody
BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) antibody
| Antigen |
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| Synonyms |
NIP3 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN343450 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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NIP3/BNIP3
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Immunogen
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A synthetic peptide corresponding to a sequence near the C-terminal of human NIP3, identical to the related mouse and rat sequence.
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Format
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Affinity-purified
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Isotype
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IgG (Matching secondary antibodies)
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Description
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The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X(L) heterodimerization and confers pro-apoptotic activity, whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.
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Specificity
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Human, mouse, rat. No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 1u/ml with the appropriate system to detect NIP3 in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
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Purity
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Immunogen affinity purified.
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Buffer
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Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Restrictions
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For Research Use only
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Alternatives for antigen "BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)", type "Antibodies"
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Hosts
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Rabbit (28)
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Reactivities
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Human (25), Mouse (Murine) (21), Rat (Rattus) (21), Chicken (2), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2), Rabbit (2)
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Applications
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Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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BH3 Domain Specific (1), pS95 (1)
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Alternatives 
