Neural Cell Adhesion Molecule 1 (NCAM1) antibody

Antigen: Neural Cell Adhesion Molecule 1 (NCAM1)

Synonyms: CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN343452

Additional Information

Alternative name: Neural cell adhesion molecule 1, NCAM1 (CD56)

Immunogen: A synthetic peptide corresponding to the C-teminal end of human CD56, different from the related rat sequence by single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: NCAM is a membrane-bound glycoprotein that plays a role in cell-cell and cell-matrix adhesion through both its homophilic and heterophilic binding activity. The neural cell adhesion molecule appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis. Later in development it is found on various differentiated tissues and is a major CAM mediating adhesion among neurons and between neurons and muscle. NCAM gene is mapped to 11q23. The neural cell adhesion molecule (NCAM) can influence a number of diverse intercellular events, including junctional communication, the association of axons with pathways and targets, and signals that alter levels of neurotransmitter enzymes.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect CD56 in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat brain tissue lysis using NCAM1 antibody