Myeloperoxidase (MPO) antibody

Antigen: Myeloperoxidase (MPO)

Synonyms: KIAA4033, mKIAA4033, mpo, fj80f04, wu:fj80f04, MPO, LOC100335032

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343453

Additional Information

Alternative name: Myeloperoxidase, MPO

Immunogen: A synthetic peptide corresponding to a sequence mapping at the C-terminal of human MPO, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2u/ml with the appropriate system to detect MPO in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 10.5-1u/ml to detect MPO in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Immunohistochemical analysis of paraffin-embedded Human tissue sections (ovary) using Myeloperoxidase antibody