Mitofusin 2 (MFN2) antibody

Antigen: Mitofusin 2 (MFN2)

Synonyms: HSG, MARF, CMT2A, CPRP1, CMT2A2, KIAA0214, Fzo, D630023P19Rik, MGC81251, MFN2, hsg, marf, cmt2a, cprp1, cmt2a2, DKFZp459G151

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN343456

Additional Information

Alternative name: Mitofusin 2, MFN2

Immunogen: A synthetic peptide corresponding to a sequence mapping at the N-terminal of human MFN2, different from the related rat and mouse sequence by single amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Mitofusin 2 (MFN2) is a mitochondrial transmembrane GTPase regulating mitochondrial fusion and that the nucleotide-dependent activation of MFN2 concomitantly protects the organelle from permeability transition. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. It is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. This protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. It shares 60% identity with MFN1. When stably expressed in COS-7 cells, MFN2 colocalizes with mitochondrial markers. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 1µg/ml, other applications:IHC-P, IHC-F, ICC dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Rat cardiac muscle tissue lysis using MFN2 antibody

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle sections. Staining MFN2 in cytoplasm and nucleusDAB chromogenic reaction