Myelin Basic Protein (MBP) antibody

Antigen: Myelin Basic Protein (MBP)

Synonyms: MGC99675, mld, shi, Hmbpr, C76307, R75289, golli-mbp, Mbps, cb274, fj33b11, MGC136630, wu:fj33b11, wu:fq15b02, zgc:136630, Mbp, MBP, MGC115135, DKFZp459G2410, DKFZp459P2220

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN343457

Additional Information

Alternative name: Myelin Basic Protein, MBP

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of the human MBP, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect MBP in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect MBP in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat brain tissue lysis using MBP antibody

Immunohistochemical analysis of paraffin-embedded Human Tissue sections (liver)Using MBP antibody