Integrin alpha 1 (ITGA1) antibody

Antigen: Integrin alpha 1 (ITGA1)

Synonyms: VLA1, CD49a, HsT18964, Vla1, CD49A, E130012M19Rik

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343462

Additional Information

Alternative name: Integrin alpha1, ITGA1

Immunogen: A peptide at the C-terminus of ITGA1 of human origin, identical to the related mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2µ/ml with the appropriate system to detect ITGA1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2µ/ml to detect ITGA1 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell lysis using ITGA1 antibody

Immunohistochemical analysis of paraffin-embedded Human pelvic cavity cancer Tissue sectionsUsing ITGA1 antibody