Heat Shock Protein 40 (HSP40) antibody

Antigen: Heat Shock Protein 40 (HSP40)

Synonyms: Hdj1, Sis1, HSPF1, Hsp40, 0610007I11Rik, hsp40

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343464

Additional Information

Alternative name: Heat Shock Protein 40, HSP40

Immunogen: A peptide mapping at the C-terminal end of human HSP40, different from the related rat and mouse sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The Hsp40 (heat shock protein with molecular size of approximately 40 kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein. Ohtsuka (1993) isolated a cDNA encoding a 40-kD heat-shock protein designated HSPF1. The deduced 340-amino acid HSPF1 protein is 34% identical to E. coli DnaJ and 34% and 36% identical to HSJ1 and HSJ2, respectively. HSPF1 gene spans over 7 kb and contains 3 exons and 2 introns. HSPF1 gene is mapped to chromosome 19p13.2.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect HSP40 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect HSP40 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell lysis using HSP40 antibody

Immunohistochemical analysis of paraffin-embedded Mammary cancer sections. Staining HSP40 in cytoplasmDAB chromogenic reaction