Glutathione S-Transferase pi 1 (GSTP1) antibody

Antigen: Glutathione S-Transferase pi 1 (GSTP1)

Synonyms: PI, DFN7, GST3, FAEES3, Gst3, Gstp, GST-P, Gstp2, MGC72668, MGC72669, GSTP1, DKFZp468B2425

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN343467

Additional Information

Alternative name: Glutathione S-Transferase pi, GST pi

Immunogen: A peptide mapping at the C-terminus of GSTpi of human origin, different from the related mouse sequence by two sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect GSTpi in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect GSTpi in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry At 0.5-1u/ml to detect GSTpi in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only