Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) antibody

Antigen: Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4)

Synonyms: GLUT4, SLC2A4, Glut4, Glut-4, MGC137703, glut4, slc2a4, MGC82597

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343468

Additional Information

Alternative name: Glucose transporter 4, GLUT4

Immunogen: A synthetic peptide corresponding to the C-terminal of human glucose transporter 4, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter . It belongs to solute carrier family 2,member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1ug/ml with the appropriate system to detect GLUT4 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2ug/ml to detect GLUT4 in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat liver section lysis using GLUT4 antibody

Immunohistochemical analysis of paraffin-embedded Human ovary cancer sectionUsing GLUT4 antibody