Glutamic Acid Decarboxylase 1 (GAD1) antibody

Antigen: Glutamic Acid Decarboxylase 1 (GAD1)

Synonyms: DGAD1, GAD, GAD1, GAD[[1]], Glb, l(3)64An, l(3)SH10, DmelCG14994, CG14994

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343471

Additional Information

Alternative name: Glutamic acid decarboxylase 67, GAD67

Immunogen: A peptide mapping very near the N-terminal end of GAD67 of human origin, identical to the related mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Glutamic acid decarboxylase (GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 585 amino acid residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594 amino acid residues. GAD67 gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD67 gene contains an additional exon (exon 0) that together with part of exon 1, specifies the 5' untranslated region of GAD67 mRNA. Human GAD67 shows 65% identity to GAD65 and is located in 2q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect GAD67 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect GAD67 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat brain tissue lysis using GAD67 antibody

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (brain)using GAD67 antibody