Endothelin Receptor Type B (EDNRB) antibody

Antigen: Endothelin Receptor Type B (EDNRB)

Synonyms: ETB, ETBR, ETRB, HSCR, ABCDS, HSCR2, s, ETb, ET-B, ET>B<, ETR-b, Sox10m1, AU022549, Etb, Ednra, MGC142327, EDNRB, etb, etrb, hscr, abcds, hscr2, MGC145633, ednrb, MGC147408

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343476

Additional Information

Alternative name: Endothelin Receptor B, EDNR-B

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human EDNR-B, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Endothelin Receptor B (EDNR-B), also called endothelin receptor, nonselective type (ETB, ETRB). It is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons and migration of melanoblasts and enteric neuroblasts. The EDNRB gene spans 24 kilobases and consists of seven exons and six introns. This gene was expressed in human glaucomatous optic nerves and mapped to 13q22. The cDNA fragment for horse EDNRB contained a 1329 bp open reading frame which encoded 443 amino acid residues. The predicted amino acid sequence was 89, 91 and 85% identical to human, bovine and mouse as well as rat EDNRB respectively, but only 55% identical to the human, bovine and rat endothelin A receptor (EDNRA). EDNRB plays an essential role in the normal development of two neural crest-derived cell lineages, epidermal melanocytes and enteric neurons in three mammalian species--humans, mice, and rats. The EDNRB-deficient rat may also prove valuable in defining the postnatal physiologic role of this receptor.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1u/ml with the appropriate system to detect EDNR-B in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect EDNR-B in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg Nacl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis ofrat intestinal cancer tissue lysis using EDNR-B antibody

Immunohistochemical analysis of paraffin-embedded Human Tissue sections (intestinal cancer)using EDNR-B antibody