Deoxyuridine Triphosphatase (DUT) antibody

Antigen: Deoxyuridine Triphosphatase (DUT)

Synonyms: DUT, anon-SAGE:Wang-077, BcDNA:LD08534, CG4584, DmelCG4584, LD08534, UTPase, Dutp, dUTPase, D2Bwg0749e, 5031412I06Rik, 5133400F09Rik, Tb07.27E10.390, dutpase

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343477

Additional Information

Alternative name: dUTP pryphosphatase, dUTPase

Immunogen: A synthetic peptide corresponding to a sequence mapping at the middle region of human dUTPase, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E. coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect dUTPase in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect dUTPase in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only