Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1) antibody

Antigen: Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1)

Synonyms: COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, Cox-1, Cox-3, Pghs1, Cox1, Cox3, PTGS1, COX-1, Ptgs1, cox1, cox-1, zCOX-1, COX-3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343480

Additional Information

Alternative name: Cyclooxygenase-1, COX1

Immunogen: A synthetic peptide corresponding to a sequence near the N-terminal of human PTGS1(COX1), different to the related rat and mouse sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Cyclooxygenase 1(COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2u/ml with the appropriate system to detect COX1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect COX1 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat testicle tissue lysis using COX1 antibody

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (spleen). Staining COX1 in cytoplasmDAB chromogenic reaction