Gap Junction Protein, beta 2, 26kDa (GJB2) antibody

Antigen: Gap Junction Protein, beta 2, 26kDa (GJB2)

Synonyms: HID, KID, PPK, CX26, DFNA3, DFNB1, NSRD1, DFNA3A, DFNB1A, Cx26, Cnx26, Gjb-2, AI325222, ppk, cx26, dfna3, dfnb1, nsrd1, dfna3a, dfnb1a, MGC53062, connexin-26, CXN-26, MGC93804, GJB2, GJA3, connexin44

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN343482

Additional Information

Alternative name: Connexin 26

Immunogen: A peptide mapping at the middle region of rat Connexin 26, different from the relative sequence of human by three amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect Connexin 26 in cells and tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only