CD40 Ligand (CD40LG) antibody

Antigen: CD40 Ligand (CD40LG)

Synonyms: IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L, Ly62, Cd40l, Ly-62, Tnfsf5, CD40LG, cd40l

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343485

Additional Information

Alternative name: CD40L

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human CD40L, different from the relative mouse sequence by three amino acids, rat sequence by four amino acids.

Format: Affinity-purified

Isotype: IgG

Description: CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB:1µg/ml for Hu, Rat,Ms IHC-P:1µg/ml Hu, Rat,Ms, By Heat IHC-F - ICC:1µg/ml Hu, Rat Ms

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2 ml of destilled water yield a concentration of 500µg/ml.

Research Area: Immunology, Adaptive Immunity, Atherosclerosis, Innate Immunity, Cytokines, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of Rat tissue sections(spleen) using CD40L antibody

Immunohistochemical analysis of paraffin-embedded Mammary cancer sections. Staining CD40L in cytoplasmDAB chromogenic reaction

Publications

Publications: Epple, Allers, Tra: "Acute HIV infection induces mucosal infiltration with CD4+ and CD8+ T cells, epithelial apoptosis, and a mucosal barrier defect." in: Gastroenterology, Vol. 139, Issue 4, pp. 1289-300, 2010 (PubMed).