CD22 Molecule (CD22) antibody
| Antigen |
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| Synonyms |
SIGLEC2, FLJ22814, SIGLEC-2, MGC130020 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
Flow Cytometry (FACS) (172), Western Blotting (WB) (30), Immunoprecipitation (IP) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), ELISA (9), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (3), Functional Studies (Func) (2)
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| Catalog no. |
ABIN343487 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD22
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Immunogen
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A peptide mapping at the C-terminal end of human CD22, different from the relative sequence of mouse by three amino acids.
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Format
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Affinity-purified
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Isotype
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IgG (Matching secondary antibodies)
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Description
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CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. CD22 is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. 3 CD22 is located within the band region q13.1 of chromosome 19.
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Specificity
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Human, mouse, rat. No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 0.5-1u/ml with the appropriate system to detect CD22 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect CD22in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1u/ml to detect CD22 in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.
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Purity
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Immunogen affinity purified.
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Buffer
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Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD22 Molecule (CD22)", type "Antibodies"
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Hosts
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Mouse (133), Rat (63), Rabbit (21)
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Reactivities
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Human (147), Mouse (Murine) (65), Primates (9), Rat (Rattus) (3), Primate (1)
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Applications
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Flow Cytometry (FACS) (172), Western Blotting (WB) (30), Immunoprecipitation (IP) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), ELISA (9), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (3), Functional Studies (Func) (2)
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Conjugates
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FITC (40), Biotin (26), PE (26), APC (12), RPE (7), SPRD (4), Cy5,PE (3), Alexa Fluor 700 (2), Cy5.5,PE (2), Pacific Blue (2), Alexa Fluor 488 (1), PE,DY647 (1)
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Epitopes
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Ab-807 (1), pTyr807 (1)
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