Chemokine (C-C Motif) Receptor 5 (CCR5) antibody

Antigen: Chemokine (C-C Motif) Receptor 5 (CCR5)

Synonyms: CKR5, CD195, CKR-5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5, FLJ78003, Ckr5, Cmkbr5, CCR5, CCR1-L, GPCR, MGC137803, CCR-5, AM4-7, CCR2, CCR5-L

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343489

Additional Information

Alternative name: C-C Chemokine Receptor 5, CCR5

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human CCR5, different from the related rat sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Human CC chemokine receptor 5 (CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. 1,3 Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. 2 Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1. 3

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect CCR5 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect CCR5 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat liver tissue lysis using CCR5 antibody

Immunohistochemical analysis of paraffin-embedded Rectal carcinoma sections. Staining CCR5 in cytoplasmDAB chromogenic reaction