ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J) antibody

Antigen: ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J)

Synonyms: F6, CF6, ATP5, ATPM, ATP5A, ATP5J, zgc:77541, wu:fa66e04, DKFZp468B1727, MGC68738, Atp5j

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343493

Additional Information

Alternative name: ATP5J

Immunogen: A synthetic peptide mapping at the middle region of human mature ATP5J (CF6), different from the related mouse sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J.This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect ATP5J in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect ATP5J in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Signaling, Metabolism, Organelles

Restrictions: For Research Use only