Aquaporin 1 (Colton Blood Group) (AQP1) antibody

Antigen: Aquaporin 1 (Colton Blood Group) (AQP1)

Synonyms: CO, CHIP28, AQP-CHIP, MGC26324, AQP1, CHIP29, MGC129064, fj33g04, zgc:85890, wu:fj33g04

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343495

Additional Information

Alternative name: Aquaporin1, AQP1

Immunogen: A synthetic peptide corresponding to a sequence mapping near the C-terminal of human AQP1, identical to the related mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Aquaporin 1 is a 28-kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules.AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect AQP1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect AQP1 in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Rat Tissue sections (kedney) lysis using AQP1 antibody

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue sections. Staining AQP1 in cytoplasmDAB chromogenic reaction