Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody

Antigen: Alkaline Phosphatase, Liver/bone/kidney (ALPL)

Synonyms: HOPS, TNAP, TNSALP, AP-TNAP, FLJ40094, FLJ93059, MGC161443, MGC167935, Akp2, Akp-2, PHOA, MGC93545, ALPL, TNS-AP, MGC139479, alpl, MGC52917, MGC130818, ALP, iap

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343501

Additional Information

Alternative name: Alkaline Phosphatase, ALPL

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human ALPL, different from the related rat and mouse sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 ug/ml with the appropriate system to detect ALPL in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 ug/ml to detect ALPL in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4 , 0.05mg Thimerosal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell tissue lysis using ALPL antibody

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (small intestine). Staining ALPL in cytoplasmDAB chromogenic reaction