Cholinergic Receptor, Nicotinic, alpha 1 (Muscle) (CHRNA1) antibody

Antigen: Cholinergic Receptor, Nicotinic, alpha 1 (Muscle) (CHRNA1)

Synonyms: ACHRA, ACHRD, CHRNA, CMS2A, FCCMS, SCCMS, Acra, Achr-1, AI385656, AI608266

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343503

Additional Information

Alternative name: Acetylcholine receptor (Nicotinic, alpha1), ACHR alpha1

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of acetylcholine receptor alpha1, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The acetylcholine receptor of muscle, like the nicotinic acetylcholine receptor of the Torpedo electric organ, has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma, and delta subunits. The alpha subunit exists in 2 isoforms. The protein-coding sequence of the human ACHRA gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule. Human nicotinic acetylcholine receptor genes alpha is assigned to chromosome 2. Mutation of the acetylcholine receptor alpha subunit causes a slow-channel myasthenic syndrome by enhancing agonist binding affinity

Specificity: Human, mouse, rat, rabbit. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 ug/ml with the appropriate system to detect ACHRalpha1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 ug/ml to detect ACHRalpha1 in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Restrictions: For Research Use only