alpha Adaptin antibody
| Antigen |
|
| Synonyms |
AP-2, AP2, Alpha-Adaptin, CG31654, D-Ada, D-alphaAda, ada, alpha-Ada, cg4260, dAP-2a, l(2)06694, DmelCG4260 |
| Clonality |
Monoclonal (Ada-1) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
|
| Catalog no. |
ABIN343514 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Alternative name
|
alpha-Adaptin
|
|
Immunogen
|
AP-2 adaptor polypeptides from bovine brain. Purification Purified by the goat anti-mouse IgG affinity chromatography.
|
|
Format
|
Affinity-purified
|
|
Isotype
|
IgG2a (Matching secondary antibodies)
|
|
Clone
|
Ada-1
|
|
Description
|
Alpha Adaptin also knows as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. . Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
|
|
Specificity
|
Human, rat. No cross reactivity with other proteins.
|
|
Application Notes
|
Western blotting: At 2-4µg/ml with the appropriate system to detect a-adaptin in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
|
|
Buffer
|
Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
|
|
Storage
|
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "alpha Adaptin", type "Antibodies"
|
Hosts
|
Rabbit (20)
|
|
Reactivities
|
Human (20), Mouse (Murine) (18), Rat (Rattus) (17), Cow (Bovine) (15), Horse (Equine) (15), Rabbit (15), Chicken (1), Frog (1), Zebrafish (Brachydanio rerio) (1)
|
|
Applications
|
Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), ELISA (3), Western Blotting (WB) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
Alternatives 
