Actinin, alpha (ACTN) antibody

Antigen: Actinin, alpha (ACTN)

Clonality: Monoclonal (SA-20)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343515

Additional Information

Alternative name: alpha-Actinin

Immunogen: Rabbit skeletal a-actinin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: SA-20

Description: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 3.5µg/ml with the appropriate system to detect a-actinin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 3.5-7µg/ml to detect a-actinin in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) At 3.5-7µg/ml to detect a-actinin in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only