Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal (VMT-24)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN343517
Quantity 100ug
Price 283.33 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Alternative name Vimentin
Immunogen Pig eye lens vimentin. Purification Purified by the goat anti-mouse IgG affinity chromatography.
Format Affinity-purified
Isotype IgG1  (Matching secondary antibodies)
Clone VMT-24
Description The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
Specificity Human, mouse, rat. No cross reactivity with other proteins.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western blotting: At 0.5-1µg/ml with the appropriate system to detect vimentin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1.5-2.5µg/ml to detect vimentin in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
Buffer Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Rabbit (95), Mouse (73), Chicken (9), Sheep (3), Guinea Pig (2), Goat (1)
Reactivities Human (156), Rat (Rattus) (65), Mouse (Murine) (59), Chicken (32), Cow (Bovine) (28), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (105), Immunofluorescence (IF) (74), Flow Cytometry (FACS) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (56), Immunohistochemistry (IHC) (55), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (16), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
Conjugates FITC (10), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitopes pSer82 (21), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)