Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody

Antigen: Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1)

Synonyms: flt1b, VEGFR1, FLT, Flt-1, sFlt1, VEGFR-1, AI323757, FLT1

Clonality: Monoclonal (V12)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343518

Additional Information

Alternative name: Vascular Endothlial Growth Factor Receptor-1, VEGFR1

Immunogen: Recombinant human VEGF-1 receptor. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: V12

Description: VEGFR1 also knows as FMS-related tyrosine kinase 1(FLT1). Oncogene FLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. FLT is mapped to 13q12. VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2µg/ml with the appropriate system to detect VEGFR-1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-4µg/ml to detect VEGFR-1 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-4µg/ml to detect VEGFR-1 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only

Images

Western blot analysis ofRat cardiac muscle tissue lysis using VEGF antibody

Immunohistochemical analysis of paraffin-embedded ESCC sections. Staining VEGF in cytoplasmDAB chromogenic reaction