Tropomyosin (sarcomeric) antibody

Antigen: Tropomyosin

Synonyms: 1305/10, 2299, BcDNA:GH09289, BcDNA:LD37158, BcDNA:SD21996, PmI, TMII, Tm, Tmr33, Tmr34, TnH, TnH-34, cTM, cTmII, l(3)02299, l(3)S130510, l(3)s2958, mTmII, region 3, tm1, DmelCG4898, CG4898, DROTROPI1, Ifm(3)3, Ifm(3)5, Ifm-TmI, Tm1, TmI, Tn-H, dro Tm, l(3)nc99Eb, mTmI, DmelCG4843, CG4843, LOC100101174

Epitope: sarcomeric

Clonality: Monoclonal (ST-39)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343522

Additional Information

Alternative name: Tropomyosin(Sarcomeric)

Immunogen: Chicken muscle tropomyosin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: ST-39

Description: Tropomyosin is an alpha-helical, parallel, two-chain coiled coil which binds along the length of actin filaments in both muscle and non-muscle cells. This gene associates N-terminus to C-terminus to form a continuous strand along both sides of the actin filament and regulates its function. Tropomyosin contributes to most, if not all, functions of the actin cytoskeleton, and its role is essential for the viability of a wide range of organisms. The ability of tropomyosin to contribute to the many functions of the actin cytoskeleton is related to the temporal and spatial regulation of expression of tropomyosin isoforms.

Specificity: Human, mouse, rat, rabbit. No cross reactivity with other proteins.

Synonyms: 1305/10, 2299, BcDNA:GH09289, BcDNA:LD37158, BcDNA:SD21996, PmI, TMII, Tm, Tmr33, Tmr34, TnH, TnH-34, cTM, cTmII, l(3)02299, l(3)S130510, l(3)s2958, mTmII, region 3, tm1, DmelCG4898, CG4898, DROTROPI1, Ifm(3)3, Ifm(3)5, Ifm-TmI, Tm1, TmI, Tn-H, dro Tm, l(3)nc99Eb, mTmI, DmelCG4843, CG4843, LOC100101174

Application Details

Application Notes: Western blotting: At 1-2µg/ml with the appropriate system to detect tropomyosin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-4µg/ml to detect tropomyosin in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-4µg/ml to detect tropomyosin in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only