Tropomyosin(36/39kDa) antibody

Antigen: Tropomyosin(36/39kDa)

Clonality: Monoclonal (TM-33)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Chicken

Application: Western Blotting (WB)

Catalog no.: ABIN343524

Additional Information

Immunogen: Chicken gizzard tropomyosin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: TM-33

Description: The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes, in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.

Specificity: Human, mouse, rat, chicken. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2-4µg/ml with the appropriate system to detect tropomyosin in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only