Spectrin, Alpha+beta- antibody

Antigen: Spectrin, Alpha+beta-

Clonality: Monoclonal (Spe 1-2)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343529

Additional Information

Alternative name: Spectrin(a and beta)

Immunogen: Human erythrocyte spectrin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: Spe 1-2

Description: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2.Spectrin mutations cause spinocerebellar ataxia type 5.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2-4µg/ml with the appropriate system to detect spectrin in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only