SMAD Family Member 4 (SMAD4) antibody

Antigen: SMAD Family Member 4 (SMAD4)

Synonyms: SMAD4, smad4

Clonality: Monoclonal (IMD-89)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN343530

Additional Information

Alternative name: Smad4(DPC4)

Immunogen: Recombinant human Smad4 (DPC4). Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: IMD-89

Description: SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2-4µg/ml with the appropriate system to detect Smad4 in cells and tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only