Proliferating Cell Nuclear Antigen (PCNA) antibody

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyms: MGC8367, PCNAR, Pcna/cyclin, pcna

Clonality: Monoclonal (IML-83)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343536

Additional Information

Alternative name: Proliferating Cell Nuclear Antigen, PCNA

Immunogen: Protein A fusion protein Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG2a

Clone: IML-83

Description: Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2 µg/ml with the appropriate system to detect PCNA in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.4-1 µg/ml to detect PCNA in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining. Immunohistochemistry(F) At 0.4-1 µg/ml to detect PCNA in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only