Keratin (KRT) (pan) antibody

Antigen: Keratin (KRT)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Epitope: pan

Clonality: Monoclonal (IML-91)

Host: Please enquire

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343538

Additional Information

Alternative name: Pan cytokeratin

Immunogen: mixture of several monoclonal cytokeratin clones. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1, IgG2a

Clone: IML-91

Description: Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Specificity: Human, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 µg/ml with the appropriate system to detect PCK in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-5 µg/ml to detect PCK in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-5 µg/ml to detect PCK in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Extracellular Matrix

Restrictions: For Research Use only