Pan-Cadherin antibody

Antigen: Pan-Cadherin

Clonality: Monoclonal (PC-79)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Chicken, Snake

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343540

Additional Information

Immunogen: Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: PC-79

Description: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma.

Specificity: Human, mouse, rat, rabbit, chicken, snake. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 µg/ml with the appropriate system to detect pan-cadherin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-4 µg/ml to detect pan-cadherin in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only