Cyclin-Dependent Kinase 1 (CDK1) antibody

Antigen: Cyclin-Dependent Kinase 1 (CDK1)

Synonyms: CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34, cdc2, MGC92032, zgc:92032, wu:fc30e01, CDC2, MGC134454, cdc-2, xcdc2, cdc2-a, cdc28a, p34cdc2, MGC53829

Clonality: Monoclonal (IMD-34)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Chicken

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343542

Additional Information

Alternative name: p34cdc2

Immunogen: C-terminal two-thirds of Xenopus p34cdc2 expressed in E. coli. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG2a

Clone: IMD-34

Description: P34(cdc2) also knows as cell division cycle(CDC2), or cyclin-dependent kinase 1(CDK1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Specificity: Human, mouse, rat, rabbit, chicken. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect p34cdc2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect p34cdc2 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect p34cdc2 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Cell Cycle

Restrictions: For Research Use only