Nitric Oxide Synthase 1 (Neuronal) (NOS1) (AA 1-181) antibody

Antigen: Nitric Oxide Synthase 1 (Neuronal) (NOS1)

Synonyms: NOS, nNOS, IHPS1, INOS, NOS2A, HEP-NOS, NO, bNOS, NOS-I, Nos-1, 2310005C01Rik, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, iNos, nos, NOS2, NOS1, nos2

Epitope: AA 1-181

Clonality: Monoclonal (N1)

Host: Please enquire

Reactivity: Human, Rat (Rattus), Goat, Pig (Porcine)

Application: Western Blotting (WB)

Catalog no.: ABIN343547

Additional Information

Alternative name: Nitric Oxide Synthase, Brain(1-181) NOS1

Immunogen: Protein A fusion protein Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: N1

Description: Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.

Specificity: Human, rat, goat, pig. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5 µg/ml with the appropriate system to detect Nitric Oxide Synthase, Brain(1-181) in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only