N-Cadherin (CD325) antibody

Antigen: N-Cadherin (CD325)

Clonality: Monoclonal (NC-17)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Chicken

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343552

Additional Information

Alternative name: N-Cadherin

Format: Affinity-purified

Isotype: IgG1

Clone: NC-17

Description: N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin, also knows as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Specificity: Human, mouse, rat, rabbit, chicken. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2 µg/ml with the appropriate system to detect N-cadherin in cells and tissues. Immunohistochemistry(F) At 4 µg/ml to detect N-cadherin in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only