Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2) antibody

Antigen: Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2)

Synonyms: HDMX, hdm2, MGC5370, MGC71221, Mdm-2, AA415488, 1700007J15Rik

Clonality: Monoclonal (Jan 02)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Catalog no.: ABIN343559

Additional Information

Alternative name: MDM2

Immunogen: Synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: Jan 02

Description: The MDM2 gene was originally identified by virtue of its amplification in a spontaneously transformed derivative of mouse BALB/c cells, and the MDM2 protein was subsequently shown to bind to p53 in rat cells transfected with p53 genes . Mdm2 acts as a major regulator of the tumor suppressor p53 by targeting its destruction. MDM2 gene is mapped to 12q14.3-q15. The mdm2 gene encodes a zinc finger protein that negatively regulates p53 function by binding and masking the p53 transcriptional activation domain. Overexpression of the MDM-2 gene in cells increases their tumorigenic potential and overcomes the growth-suppressive activity of p53.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect MDM2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect MDM2 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect MDM2 in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Cancer, Cell Cycle, Transcription Factors

Restrictions: For Research Use only