MAP-1 antibody

Antigen: MAP-1

Clonality: Monoclonal (MP-1)

Host: Please enquire

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343562

Additional Information

Alternative name: MAP1

Immunogen: Rat brain microtubule-associated proteins (MAPs) Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: MP-1

Description: Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins(MAPs). MAP1 is one of the major neuronal MAPs as well as being the largest(350KD). MAPs include MAP1A, MAP1B , and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.

Specificity: Rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-2 µg/ml with the appropriate system to detect MAP1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect MAP1 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect MAP1 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only