MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody

Antigen: MAP Kinase, Activated (Diphosphorylated ERK-1&2)

Clonality: Monoclonal (IL-13)

Host: Please enquire

Reactivity: Human, Insect, Mouse (Murine), Rat (Rattus), Yeast (Saccharomyces Cerevisiae)

Application: Western Blotting (WB)

Catalog no.: ABIN343563

Additional Information

Format: Affinity-purified

Isotype: IgG1

Clone: IL-13

Description: In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Specificity: Human, insect, mouse, rat, yeast. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.25-0.5 µg/ml with the appropriate system to detect MAP kinase, activated (Diphosphorylated ERK-1&2) in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Storage (Long Term): At -20°C for one year. It can also be aliquotted and stored frozen at -20°C for longer time.

Storage (Short Term): After reconstitution, at 4°C for one month.

Restrictions: For Research Use only