Heat Shock Protein 70 (HSP70) (HSPA4) antibody

Antigen: Heat Shock Protein 70 (HSP70) (HSPA4)

Clonality: Monoclonal (SJ-70)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343567

Additional Information

Alternative name: Heat Shock Protein 70, HSP70

Immunogen: HSP70 isolated from bovine brain. Purification Purified by the goat anti-mouse IgG affinity chromatography. Application

Format: Affinity-purified

Isotype: IgG1

Clone: SJ-70

Description: Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. (1989) identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5 µg/ml with the appropriate system to detect HSP70 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1 µg/ml to detect HSP70 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1 µg/ml to detect HSP70 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only