Heat Shock Protein 25 (HSP25) antibody

Antigen: Heat Shock Protein 25 (HSP25)

Synonyms: HSP25, LOC100231347

Clonality: Monoclonal (SJ-25)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343569

Additional Information

Alternative name: Heat Shock Protein 25, HSP25

Immunogen: Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: SJ-25

Description: The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-2 µg/ml with the appropriate system to detect HSP25 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect HSP25 in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining. Immunohistochemistry(F) At 1-2 µg/ml to detect HSP25 in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only