Gelsolin (GSN) antibody

Antigen: Gelsolin (GSN)

Synonyms: DKFZp313L0718, gsn, cb499, gsnl1, fb94b12, fc04g09, sb:cb499, wu:fc04g09, GSN, DKFZp469O1525, DGS, gel, DmelCG1106, CG1106, MGC28083, MGC95032, LOC397895, MGC79133, MGC127421, scin, gelsolin

Clonality: Monoclonal (GEL-42)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343573

Additional Information

Alternative name: Gelsolin

Immunogen: Human plasma gelsolin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: GEL-42

Description: Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain

Specificity: Human, mouse, rat, rabbit. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect gelsolin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect gelsolin in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At1-2 µg/ml to detect gelsolin in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only