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Antibodies
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anti-Growth Associated Protein 43 (GAP43) antibody
Growth Associated Protein 43 (GAP43) antibody
| Antigen |
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| Synonyms |
B-50, PP46, Basp2, GAP-43, gap-43, MGC64520 |
| Clonality |
Monoclonal (GAP-8A12) |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Chicken, Snake
Rat (Rattus) (61), Human (50), Mouse (Murine) (44), Chicken (23), Cow (Bovine) (10), Rabbit (7), Dog (Canine) (5), Mammalian (3), Pig (Porcine) (3), Xenopus laevis (2), Bee (Apiformes) (1), Cat (Feline) (1), Finch (1), Frog (1), Guinea Pig (1), Horse (Equine) (1), Monkey (1), Silk Worm (1), Zebrafish (Danio rerio) (1)
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Western Blotting (WB) (51), Immunofluorescence (IF) (44), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (10), ELISA (9), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN343575 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Growth Associated Protein-43, GAP43
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Immunogen
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GAP-43 from neonatal rat forebrain membranes. Purification Purified by the goat anti-mouse IgG affinity chromatography.
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Format
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Affinity-purified
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Isotype
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IgG2a
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Clone
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GAP-8A12
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Description
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GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
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Specificity
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Human, mouse, rat, chicken, snake No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 0.5-1 µg/ml with the appropriate system to detect GAP43 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect GAP43 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect GAP43 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
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Buffer
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Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.
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Restrictions
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For Research Use only
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Alternatives for antigen "Growth Associated Protein 43 (GAP43)", type "Antibodies"
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Hosts
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Rabbit (70), Mouse (10), Sheep (7), Chicken (1)
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Reactivities
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Rat (Rattus) (61), Human (50), Mouse (Murine) (44), Chicken (23), Cow (Bovine) (10), Rabbit (7), Dog (Canine) (5), Mammalian (3), Pig (Porcine) (3), Xenopus laevis (2), Bee (Apiformes) (1), Cat (Feline) (1), Finch (1), Frog (1), Guinea Pig (1), Horse (Equine) (1), Monkey (1), Silk Worm (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (51), Immunofluorescence (IF) (44), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (10), ELISA (9), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (2), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer41 (10), pS41 (4), AA 216-226 (1), N-Term (1), N-Term part (1)
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