Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) antibody

Antigen: Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)

Synonyms: Flk1, Ly73, Flk-1, Krd-1, VEGFR2, VEGFR-2, sVEGFR-2, 6130401C07, FLK1, CD309, VEGFR, Vegfr-2, MGC93590, KDR, FLK-1, flk-1, vegfr-2

Clonality: Monoclonal (FLK-6)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343577

Additional Information

Alternative name: Vascular Endothlial Growth Factor, VEGF-R2

Immunogen: Recombinant human extracellular domain of VEGFR-2 (KDR). Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: FLK-6

Description: VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family.Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect VEGFR2 in cells and tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect VEGFR2 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only

Images

Western blot analysis ofRat cardiac muscle tissue lysis using VEGF antibody

Immunohistochemical analysis of paraffin-embedded ESCC sections. Staining VEGF in cytoplasmDAB chromogenic reaction