Collagen, Type IV (COL4) antibody

Antigen: Collagen, Type IV (COL4)

Synonyms: 1209, 6072, CT25584, DmColA2, alpha(IV)2/vkg, coll. IV, l(2)01209, DmelCG16858, CG16858, CG25C, CT12803, Cgc25, Cgc25C, DCg1, Dcg, alpha(IV)1/Cg25C, alpha1, gp125, DmelCG4145, CG4145

Clonality: Monoclonal (Col-30)

Host: Please enquire

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343587

Additional Information

Alternative name: Collagen, Type IV

Immunogen: Human collagen type IV Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: Col-30

Description: Collagen IV is comprised of a third chain (alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.

Specificity: Human, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1 µg/ml to detect collagen type IV in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1 µg/ml to detect collagen type IV in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only