Collagen, Type III (COL3) antibody

Antigen: Collagen, Type III (COL3)

Clonality: Monoclonal (Col-29)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343588

Additional Information

Alternative name: Collagen, Type III

Immunogen: Human collagen type ? Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: Col-29

Description: Collagen type ?, also knows as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.25-0.5 µg/ml with the appropriate system to detect collagen type ?in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1 µg/ml to detect collagen type ? in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) At 0.5-1 µg/ml to detect collagen type ?in formalin/acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only