Cytokeratin Peptide 4 antibody

Antigen: Cytokeratin Peptide 4

Clonality: Monoclonal (CK-4)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343590

Additional Information

Immunogen: Cytokeratin from human esophagus. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: CK-4

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 µg/ml with the appropriate system to detect cytokeratin peptide 4 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-4 µg/ml to detect cytokeratin peptide 4 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-4 µg/ml to detect PCNA in formalin/acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only