Carcinoembryonic Antigen (CEA) antibody

Antigen: Carcinoembryonic Antigen (CEA)

Synonyms: CEACAM5, CEA, CEACAM, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik

Clonality: Monoclonal (CEA-9)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343594

Additional Information

Alternative name: Carcinoembryonic Antigen, CEA

Immunogen: Carcinoembryonic antigen(CEA) isolated from a human colon adenocarcinoma cell line. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: CEA-9

Description: Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect CEA in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-2 µg/ml to detect CEA in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only