»
Antibodies
»
anti-Cyclin-Dependent Kinase 4 (CDK4) antibody
Cyclin-Dependent Kinase 4 (CDK4) antibody
| Antigen |
|
| Synonyms |
CMM3, PSK-J3, MGC14458, 8-6, CDK4, CDK4/6, DmCdk4, Pk53C, Pk?7, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, DmelCG5072, CG5072, MGC133903 |
| Clonality |
Monoclonal (IML-4) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
Western Blotting (WB) (90), Immunofluorescence (IF) (50), Immunoprecipitation (IP) (46), Flow Cytometry (FACS) (22), Enzyme Immunoassay (EIA) (16), ELISA (13), Functional Studies (Func) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (4), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN343597 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Alternative name
|
CDK4
|
|
Immunogen
|
Recombinant human Cdk4 protein. Purification Purified by the goat anti-mouse IgG affinity chromatography.
|
|
Format
|
Affinity-purified
|
|
Isotype
|
IgG2a
|
|
Clone
|
IML-4
|
|
Description
|
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
|
|
Specificity
|
Human, mouse, rat. No cross reactivity with other proteins.
|
|
Application Notes
|
Western blotting: At 0.5-1 µg/ml with the appropriate system to detect Cdk4 in cells and tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.
|
|
Buffer
|
Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
|
|
Storage
|
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.
|
|
Research Area
|
Cell Cycle
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Cyclin-Dependent Kinase 4 (CDK4)", type "Antibodies"
|
Hosts
|
Mouse (61), Rabbit (47), Sheep (2)
|
|
Reactivities
|
Human (101), Mouse (Murine) (29), Rat (Rattus) (27), Pig (Porcine) (17), Cow (Bovine) (16), Dog (Canine) (2), Horse (Equine) (2), Rabbit (1)
|
|
Applications
|
Western Blotting (WB) (90), Immunofluorescence (IF) (50), Immunoprecipitation (IP) (46), Flow Cytometry (FACS) (22), Enzyme Immunoassay (EIA) (16), ELISA (13), Functional Studies (Func) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (4), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
C-Term (3), Center C215 (2)
|
